Publications (2000 -> ...)

DE CLERCQ N., VANDEN BUSSCHE J., VANMEULEBROEK L., CROUBELS S., DELAHAUT P., BUYST D., MARTINS J., STAHL-ZENG J., VANHAECKE L.
Metabolic fingerprinting reveals a novel candidate biomarker for prednisolone treatment in cattle.
Metabolomics, 2015, doi: 10.1007/s11306-015-0887-3.

DE CLERCQ N., VAN MEULEBROEK L., VANDEN BUSSCHE J., CROUBELS S., DELAHAUT P., VANHAECKE L.
The impact of stress on the prevalence of prednisolone in bovine urine : A metabolic fingerprinting approach.
Journal of Steroid Biochemistry & Molecular Biology, 2015, 154, 206-216, doi: 10.1016/j.jsbmb.2015.08.026.

PHAM D. K., CHU J., THUY DO NGA, BROSE F., DEGAND G., DELAHAUT P., DE PAUW E., DOUNY C., VAN NGUYEN K., VU T. D., SCIPPO M-L , HEIMAN W. F. L.
Monitoring Antibiotic Use and Residue in Freshwater Aquaculture for Domestic Use in Vietnam.
EcoHealth, 2015, doi: 10.1007/s10393-014-1006-z.

ROBERT C., GILLARD N., BRASSEUR P-Y, RALET N., DUBOIS M., DELAHAUT P.
Rapid multiresidue and multi-class screening for antibiotics and benzimidazoles in feed by ultra high performance liquid chromatography coupled to tandem mass spectrometry.
Food Control, 2015, 50, 509-515.

THOMAS A., WALPURGIS K., TRETZEL L., BRINKKÖTTER P., FICHANT E., DELAHAUT P., SCHÄNZER W., THEVIS M.
Expanded test method for peptides >2 kDa employing immunoaffinity purification and LC-HRMS/MS.
Drug Test. Analysis, 2015, doi: 10.1002/dta.1868.
 
TRETZEL L.,  THOMAS A., GEYER H., DELAHAUT P., SCHÄNZER W., THEVIS M.
Determination of Synacthen® in dried blood spots for doping control analysis using liquid chromatography tandem mass spectrometry.
Anal. Bioanal. Chem., 2015, 407, 4709-4720, doi: 10.1007/s00216-015-8674-6.

WAUTERS J., VANDEN BUSSCHE J., LE BIZEC B., KIEBOOMS J. A. L. , DERVILLY-PINEL G., PREVOST S., WOZNIAK B., STERK S.S., GRØNNINGEN D., KENNEDY D. G. , RUSSELL S., DELAHAUT P., VANHAECKE L.
Towards a New European Threshold to Discriminate Illegally Administered from Naturally Occurring Thiouracil in Livestock.
J. Agric. Food Chem., 2015, 63 (5), 1339–1346, doi: 10.1021/jf504475f.

CLARKE L., FODEY T.L., CROOKS S.R.H., MOLONEY M., O'MAHONY J., DELAHAUT P., O'KENNEDY R., DANAHER M.
A review of coccidiostats and the analysis of their residues in meat and other food.
Meat Science, 2014, 97, 358-374.

DE CLERCQ N., VANDEN BUSSCHE J., CROUBELS S., DELAHAUT P., VANHAECKE L.
Development and validation of a high-resolution mass-spectrometry-based method to study the long-term stability of natural and synthetic glucocorticoids in faeces.
Journal of Chromatography A, 2014, 1336, 76-86.

GILLARD N., DETRY B., ROBERT C., NONET S., SAMOU Y. , MOISE E. , BAUWENS F. , DELAHAUT P.
Detection of pharmaceutical products in untreated hospital wastewater.
Quality Assurance and Safety of Crops & Foods, 2014, volume 6, number 4, 459-467, doi: 10.3920/QAS2012.0177.

HÖPPNER S., DELAHAUT P., SCHÄNZER W., THEVIS M.
Mass spectrometric studies on the in vivo metabolism and excretion of SIRT1 activating drugs in rat urine, dried blood spots, and plasma samples for doping control purposes.
Journal of Pharmaceutical and Biomedical Analysis, 2014, 88, 649-659.

JOHNSON E.P., RIGBY N.M., DAINTY J.R., MACKIE A.R., IMMER U.U., ROGERS A., TITCHENER P., SHOJI M., RYAN A., MATA L., BROWN H., HOLZHAUSER T., DUMONT V., WYKES J.A., WALKER M., GRIFFIN J., WHITE J., TAYLOR G., POPPING B. CREVEL R., MIGUEL S., LUTTER P., GASKIN F., KOERNER T.B., CLARKE D., SHERLOCK R., FLANAGAN A., CHUN-HAN C., MILLS E.N.C.    A multi-laboratory evaluation of a clinically-validated incurred quality control material for analysis of allergens in food.    Food Chemistry, 2014, 148, 30-36
OTTO G., DELAHAUT P.
Tracking down allergens in food : a review of available methods.
Food Science & Law, 2014, 2014/4, 199-209.

THEVIS M., THOMAS A., PIPER T., KRUG O., DELAHAUT P, SCHÄNZER W.
Liquid chromatography-high resolution/high accuracy (tandem) mass spectrometry-based identification in vivo generated metabolites of the selective androgen receptor modulator ACP-105 for doping control purposes.
Eur. J. Mass Spectrom., 2014, 20, 73-83.

BIENENMANN-PLOUM M., VINCENT U., HUET A.-C., CAMPBELL K., HAASNOOT W., DELAHAUT P., HOOIJERINK D., ELLIOTT C., NIELEN M.
Single-laboratory validation of a multiplex flow cytometric immunossay for the simultaneous detection of coccidiostats in eggs and feed.
Analytical & Bioanalytical Chemistry, 2013, doi: 10.1007/s00216-013-7362-7.

DE CLERCQ N., VANDEN BUSSCHE J., CROUBELS S., DELAHAUT P., VANHAECKE L.    
A validated analytical method to study the long-term stability of natural and synthetic glucocorticoids in livestock urine during ultra-high performance liquid chromatography coupled to Orbitrap-high resolution mass spectrometry.
Journal of Chromatography A, 2013, 1301, 111-121.

DELAHAUT P., DEMOULIN L., GILLARD N., FICHANT E., COURTHEYN D.    
Preliminary study on the presence of prednisolone in porcine urine and liver - How to distinguish endogenous from therapeutically administered prednisolone.
Drug Testing and Analysis, 2013,
(www.drugtestinganalysis.com) doi: 10.1002/dta.1478.

EL AMIRI B., DELAHAUT P., COLEMONTS Y., MELO DE SOUSA N., BECKERS J.-F.    
Investigation of pregnancy-associated glycoproteins (PAGs) by means of an enzymoimmunoassay (ELISA) sandwich kit for pregnancy monitoring in sheep.
Options Méditerranéennes, A, 2013, 108, 299-303.

GURMIT S., BRADY B., KOERNER T., BECALSKI A., ZHAO T., FENG S., GODEFROY S. B., HUET A.-C., DELAHAUT P.
Development of a highly sensitive competitive indirect enzyme-linked immunosorbent assay for the detection of acrylamide in foods and water.
Food. Anal. Methods, 2013, doi: 10.1007/s12161-013-9749-7.

HUET A.-C., BIENENMANN-PLOUM M., VINCENT U., DELAHAUT P.
Screening methods and recent developments in the detection of anticoccidials.
Anal. Bioanal. Chem., 2013, 405, 7733-7751.

LINSINGER T.P.J., CHAUDHRY Q., DEHALU V., DELAHAUT P., DUDKIEWICZ A., GROMBE R., von der KAMMER F., LARSEN E.H., LEGROS S., LOESCHNER K., PETERS R., RAMSCH R., ROEBBEN G., TIEDE K., WEIGEL S.     Validation of methods for the detection and quantification of engineered nanoparticles in food.    Food Chemistry, 2013, 138, 1959-1966.
McGRATH T.F., BUIJS J., HUET A.-C., DELAHAUT P., ELLIOTT C.T., MOONEY M.H.
Assessment of a multiplexing high throughput immunochemical SPR biosensor in measuring multiple proteins on a single biosensor chip.
Sensors and Actuators B, 2013, 186, 423-430.

OPLATOWSKA M., ELLIOTT C.T., HUET A.-C., McCARTHY M., MULDER P.P.J., VON HOSLT C., DELAHAUT P., VAN EGMOND H.P., CAMPBELL K.
Development and validation of a rapid multiplex ELISA for pyrrolizidine alkaloids and their N-oxides in honey and feed.
Anal. Bioanal. Chem., 2013, doi: 10.1007/s00216-013-7488-7.

ROBERT C., GILLARD N., BRASSEUR P.-Y., PIERRET G., RALET N., DUBOIS M., DELAHAUT P.
Rapid multi-residue and multi-class qualitative screening for veterinary drugs in foods of animal origin by UHPLC-MS/MS.
Food Additives & Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment, 03/2013, 30, 3, doi: 10.1080/19440049.2012.751632.

SUAREZ PANTALEON C., HUET A.-C., KAVANAGH O., LEI H., DERVILLY-PINEL G.,
LE BIZEC B., SITU C., DELAHAUT P.
Production of polyclonal antibodies directed to recombinant methionyl bovine somatotropin.
Analytica Chimica Acta, 2013, 761, 186-193.

THOMAS A., WALPURGIS K., DELAHAUT P., KOHLER M., SCHÄNZER W., THEVIS M.
Detection of small interfering RNA (siRNA) by mass spectrometry procedures in doping controls.
Drug Test. Analysis, 2013, (www.drugtestinganalysis.com) doi: 10.1002/dta.1519.

BIENENMANN-PLOUM M. E., HUET A.-C., CAMPBELL K., TERENCE L. F., URSULA V., HAASNOOT W., DELAHAUT P., ELLIOTT C., NIELEN M. W. F.
Development of a five-plex flow cytometric immunoassay for the simultaneous detection of six coccidiostats in feed and eggs.
Anal. Bioanal. Chem., 2012, 404, 1361-1373, doi: 10.1007/s00216-012-6214-1.

DECLOEDT A., VERHEYEN T, DE CLERCQ D., SYS S., VERCAUTEREN G, DUCATELLE R., DELAHAUT P., van LOON G.
Acute and long-term cardiomyophathy and delayed neurotoxicity after accidental lasalocid poisoning in horses.
J. Vet. Intern. Med., 2012, 26, 1005-1011.

DEHALU V., WEIGEL S., REBE S., GROMBE R., LÖBENBERG R., DELAHAUT P.
Production and characterization of antibodies against crosslinked gelatin nanoparticles and first step towards developing an ELISA screening kit.
Anal. Bioanal. Chem., 2012, 403, 2851-2857.

DELAHAUT P.
Immunoassays for detecting prohibited growth promoters in the food chain.
Bentham Science Publishers, E-Books, Editor : Eline Meulenberg,
eISBN : 978-1-60805-264-6, 2012.

GEORGE S., ELLIOTT C., McLAUGHLIN D., DELAHAUT P., AKAGI T., AKASHI M.,
FODEY T.
An investigation into the potential use of nanoparticles as adjuvants for the production of antibodies to low molecular weigth food contaminants.
Veterinary Immunology and Immunopathology, 2012, 149, 46-53.

LATTANZIO V.M.T., NIVARLET N., LIPPOLIS V., DELLA GATTA S., HUET A.-C., DELAHAUT P., GRANIER B., VISCONTI A.
Multiplex dipstick immunoassay for semi-quantitative determination of Fusarium mycotoxins in cereals.
Analytica Chimica Acta, 2012, 718, 99-108.

McNAMEE S. E., ELLIOTT C. T., DELAHAUT P., CAMPBELL K.
Multiplex biotoxin surface plasmon resonance method for marine biotoxins in algal and seawater samples.
Environ. Sci. Pollut. Res., published on line - Dec. 19 2012, doi: 10.1007/s11356-012-1329-7.

MÖLLER I., THOMAS A., DELAHAUT P., GEYER H., SCHÄNZER W., THEVIS M.
Mass spectrometric detection of peginesatide in human urine in doping control analysis.
Journal of Pharmaceutical and Biomedical Analysis, 2012, 70, 512-517.

THOMAS A., DELAHAUT P., KRUG O., SCHÄNZER W., THEVIS M.
Metabolism of Growth Hormone Releasing Peptides.
Anal. Chem., 2012, 84, 10252-10259, doi: 10.1021/ac302034w.

THOMAS A., SCHÄNZER W., DELAHAUT P., THEVIS M.
Immunoaffinity purification of peptide hormones prior to liquid chromatography-mass spectrometry in doping controls.
Methods, 2012, 56, 230-235.

VANDENBERGE V., DELEZIE E., HUYGHEBAERT G., DELAHAUT P., DAESELEIRE E., CROUBELS S.
Residues of sulfadiazine and doxycycline in broiler liver and muscle tissue due to cross-contamination of feed.
Food Additives and Contaminants, February 2012, 29, 2, 180-188.

VANDENBERGE V., DELEZIE E., HUYGHEBAERT G., DELAHAUT P., DE BACKER P., DAESELEIRE E., CROUBELS S.
Residues of sulfadiazine and doxycycline in egg matrices due to cross-contamination in the feed of laying hens and the possible correlation with physicochemical, pharmacokinetic and physiological parameters.
Food Additives and Contaminants, 2012, iFirst, 1-10.

VANDENBERGE V., DELEZIE E., HUYGHEBAERT G., DELAHAUT P., PIERRET G., DE BACKER P., CROUBELS S., DAESELEIRE E.
Transfer of the coccidiostats monensin and lasalocid from feed at cross-contamination levels to whole egg, egg white and egg yolk.
Food Additives and Contaminants, 2012, i-First, doi: 10.1080/19440049.2012.719641.

SINGH G., GELINAS J.M., ABBOTT M., KOERNER T.B., GODEFROY S.B., HUET A-C., CHARLIER C., DELAHAUT P.
Design and characterization of a direct ELISA for the detection and quantification of leucomalachite green.
Food Additives and Contaminants, June 2011, 28, 6, 731-739.

ABBOTT M., HAYWARD S., ROSS W., GODEFROY S.B., ULBERTH F., Van HENGEL A.J., ROBERTS J., AKIYAMA H., POPPING B., YEUNG J.M., WEHLING P., TAYLOR S.L., POMS R.E., DELAHAUT P.
Validation procedures for quantitative food allergen ELISA methods : community guidance and best practices.
Journal of AOAC International, 2010, 93, 2, 442-450.

DELAHAUT P., PIERRET G., RALET N., DUBOIS M., GILLARD N.
Multi-residue method for detecting coccidiostats at carry-over level in feed by HPLC-MS/MS.
Food Additives and Contaminants, June 2010, 27, 6, 801-809.

DUBOIS M., DEMOULIN L., CHARLIER C., SINGH G., GODEFROY S.B., CAMPBELL K., ELLIOTT C.T., DELAHAUT P.
Development of ELISAs for detecting domoic acid, okadaic acid, and saxitoxin and their applicability for the detection of marine toxins in samples collected in Belgium.
Food Additives and Contaminants, June 2010, 27, 6, 859-868.

DUMONT V., DELAHAUT P.
Processing of egg-free foods.    
Allergen Management in the Food Industry, John Wiley & Sons, Inc., Editors :
Joyce I. Boye & Samuel Benrejeb Godefroy, Chap. 9, 2010.

DUMONT V., KERBACH S., POMS R., JOHNSON P., MILLS C., POPPING B.,
TÖMÖSKÖZI S., DELAHAUT P.
Development of milk and egg incurred reference materials for the validation of food allergen detection methods.
Quality Assurance and Safety of Crops & Foods, 2010, 2, 208-215.

HUET A.-C., FODEY T., HAUGHEY S.A., WEIGEL S., ELLIOTT C., DELAHAUT P.
Advances in biosensor-based analysis for antimicrobial residues in foods.
Trends in Analytical Chemistry, December 2010, 29, 11, 1281-1294.

NEGRO S.S., CAUDRON A.K., DUBOIS M., DELAHAUT P., GEMMELL N.J.
Correlation between male social status, testosterone levels, and parasitism in a dimorphic polygynous mammal.
PLOS ONE http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938340/
September 2010, Volume 5, Issue 9, e12507.

POLENTA G., GODEFROY-BENREJEB S., DELAHAUT P., WEBER D., ABBOTT M.
Development of a Competitive ELISA for the Detection of Pecan (Carya illinoinensis (Wangenh.) K. Koch) Traces in Food.
Food Anal. Methods, 2010, 3, 375-381, doi: 10.1007/s12161-009-9075-2.

THOMAS A., KOHLER M., DELAHAUT P., SCHÄNZER W., THEVIS M.
Preliminary results for the development of a peptide screening method by means of LC-MS/MS.
Sportverlag Strauss, Proceedings of the Manfred Donike Workshop, 28th Cologne Workshop on Dope Analysis, Recent advances in doping analysis (18), Köln 2010.
 
THOMAS A., KOHLER M., SCHÄNZER W., DELAHAUT P., THEVIS M.
Determination of IGF-1 and IGF-2, their degradation products and synthetic analogues in urine by LC-MS/MS.
Analyst, 2010, doi: 10.1039/c0an00632g.

CACCIATORE G., EISENBERG S.W.F., SITU C., MOONEY M.H., DELAHAUT P., KLARENDEEK S., HUET A.-C., BERGWERFF A.A., ELLIOTT C.
Effect of growth-promoting 17beta-estradiol, 19-nortestosterone and dexamethasone on circulating levels of nine potential biomarker candidates in veal calves.
Analytica Chimica Acta, 2009, 637, 351-359.

CAMPBELL K., HUET A.-C., CHARLIER C., HIGGINS C., DELAHAUT P., ELLIOTT T. C.
Comparison of ELISA and SPR biosensor technology for the detection of paralytic shellfish poisoning toxins.
Journal of Chromatography B, 2009, 877, 4079-4089.

GILLARD N., AGNEESSENS R., DUBOIS M.L., DELAHAUT P.
Quantification of patulin in Belgian handicraft-made apple juices.
World Mycotoxin Journal, 2009, 2 (1), 95-104.

HUET A.-C., CHARLIER C., WEIGEL S., BENREJEB GODEFROY S., DELAHAUT P.
Validation of an optical surface plasmon resonance biosensor assay for screening (fluoro)quinolones in egg, fish and poultry.
Food Additives and Contaminants, October 2009, 26, 10, 1341-1347.

THOMAS A., KOHLER M., SCHÄNZER W., KAMBER M., DELAHAUT P., THEVIS M.
Determination of Synachten in urine for sports drug testing by means of nano-ultra-performance liquid chromatography/tandem mass spectrometry.
Rapid Commun. Mass Spectrom., 2009, 23, 2669-2674.

THOMAS A., SCHÄNZER W., DELAHAUT P., THEVIS M.
Sensitive and fast identification of urinary human, synthetic and animal insulin by means of nano-HPLC coupled with high-resolution/high-accuracy mass spectrometry.    
Drug Testing and Analysis, 2009, (www.drugtestinganalysis.com)
doi: 10.1002/dta.35.

TRIPP K.M., DUBOIS M., DELAHAUT P., VERSTEGEN J.P.
Detection and identification of plasma progesterone metabolites in the female Florida manatee (Trichechus manatus latirostris).
Theriogenology, 2009, 72, 365-371.

WEIGEL S., PIKKEMAAT M.G, ELFERINK J.W.A., MULDER P.P.J., HUET A.-C., DELAHAUT P., SCHITTKO S., FLERUS R., NIELEN M.W.F.
Comparison of a fluoroquinolone surface plasmon resonance biosensor screening assay with established methods.
Food Additives and Contaminants, April 2009, 26(4), 441-452.

FODEY T., DELAHAUT P., CHARLIER C., ELLIOTT C.
Comparison of three adjuvants used to produce polyclonal antibodies to veterinary drugs.
Veterinary Immunology and Immunopathology, 2008, 122, 25-34.

HUET A.-C., CHARLIER C., SINGH G., BENREJEB GODEFROY S., LEIVO J., VEHNIÄINEN M., NIELEN M.W.F., WEIGEL S., DELAHAUT P.
Development of an optical surface plasmon resonance biosensor assay for (fluoro)quinolones in egg, fish, and poultry meat.
Analytica Chimica Acta, 2008, 623, 195-203.

KUURANNE T., THOMAS A., LEINONEN A., DELAHAUT P., BOSSELOIR A.,
SCHÄNZER W., THEVIS M.
Insulins in equine urine : qualitative analysis by immunoaffinity purification and liquid chromatography/tandem mass spectrometry for doping control purposes in horse-racing.
Rapid Commun. Mass Spectrom., 2008, 22, 355-362.

FODEY T.L., DELAHAUT P., ELLIOTT C.
Use of antigen mimics to produce specific antibodies to anti-coccidial drugs.
Journal of Immunological Methods, 2007, 323, 31-38.

MARCHESINI G.R., HAASNOOT W., DELAHAUT P., GERCEK H., NIELEN M.W.F.
Dual biosensor immunoassay-directed identification of fluoroquinolones in chicken muscle by liquid chromatography electrospray time-of-flight mass spectrometry.
Analytica Chimica Acta, 2007, 586, 259-268.

MASSIP L., ECTORS F., DEPREZ P., MALEKI M., BEHETS C., LENGELE B., DELAHAUT P., PICARD J., REZSÖHAZY R.
Expression of Hoxa2 in cells entering chondrogenesis impairs overall cartilage development.
Differentiation, 2007, 75, 256-267.

VAN SOOM A., IMBERECHTS H., DELAHAUT P., THIRY E., VAN ROY V., WALRAVENS K., ROELS S., SAEGERMAN C.
Sanitary control in bovine embryo transfer - How far should we go ? A review.    
Veterinary Quarterly Publications on Animal Diseases, 2007, 29, number 1, 2-17.

CACCIATORE G., SITU C., MERCKX T., MOONEY M., ELSENBERG S., DELAHAUT P., ELLIOTT C., DE PAUW E., BERGWERFF A.
Biomarkers revealing abuse of steroid hormones.
Journal of Veterinary Pharmacology and Therapeutics, 2006, 29 Suppl. 1, 143-144.

DAESELEIRE E., HUET A.-C., HUYGHEBAERT G., DELAHAUT P.    
Case study : coccidiostats.
Scientific Support Plan for a Sustainable Development Policy, Supporting Actions
Platform for Scientific Concertation : Food Safety
Towards a Safer Food Supply in Europe
SPSD II, 2000-2006, 190-205.

DUMONT V., HUET A.-C., TRAYNOR I., ELLIOTT C., DELAHAUT P.
A surface plasmon resonance biosensor assay for the simultaneous determination of thiamphenicol, florefenicol, florefenicol amine and chloramphenicol residues in shrimps.
Analytica Chimica Acta, 2006, 567, 179-183.

HUET A.-C., CHARLIER C., DAESELEIRE E., DELAHAUT P.
Development of immunoassays for detecting the coccidiostats halofuginone, nicarbazin and nitroimidazoles in egg and chicken muscle.
Scientific Support Plan for a Sustainable Development Policy, Supporting Actions
Platform for Scientific Concertation : Food Safety
Towards a Safer Food Supply in Europe
SPSD II, 2000-2006, 177-189.

HUET A.-C., CHARLIER C., TITTLEMIER S.A., BEN REJEB S., DELAHAUT P.
Simultaneous determination of (fluoro)quinolone antibiotics in kidney, marine products, eggs and muscle by ELISA.
J. Agric. Food Chem., 2006, 54, 2822-2827.

MHADHBI H., BEN-REJEB S., CLEROUX C., MARTEL A., DELAHAUT P.
Generation and characterization of polyclonal antibodies against microcystins - Application to immunoassays and immunoaffinity sample preparation prior to analysis by liquid chromatography and UV detection.
Talanta, 2006, 70, 225-235.

THEVIS M., BREDEHÖFT M., GEYER H., KAMBER M., DEHALAUT P., SCHÄNZER W.
Determination of Synacthen in human plasma using immunoaffinity purification and liquid chromatography/tandem mass spectrometry.
Rapid Commun. Mass Spectrom., 2006, 20, 3551-3556.

THEVIS M., THOMAS A., DELAHAUT P., BOSSELOIR A., SCHÄNZER W.
Doping control analysis of intact rapid-acting insulin analogues in human urine by liquid chromatography-tandem mass spectrometry.
Anal. Chem., 2006, 78, 1897-1903.

BEN REJEB S., ABBOTT M., DAVIES D., CLEROUX C., DELAHAUT P.
Multi-allergen screening immunoassay for the detection of protein markers of peanut and four tree nuts in chocolate.
Food Additives and Contaminants, August 2005, 22(8), 709-715.

DAESELEIRE E., DELAHAUT P., HUYGHEBAERT G.
Integrated approach for the control on residues of coccidiostats in eggs. Project CP/29.
Final Report
SPSD II, Part 1 : Sustainable production and consumption patterns, May 2005.


DELAHAUT P.
Méthodes d'analyses immunochimiques pour le contrôle de qualité dans les IAA - Chapitre 6 : Hormones.
Lavoisier, 2005, 197-220.

HUET A.-C., MORTIER L., DAESELEIRE E., FODEY T. , ELLIOTT C., DELAHAUT P.
Development of an ELISA screening test for nitroimidazoles in egg and chicken muscle.
Analytica Chimica Acta, 2005, 534, 157-162.

HUET A.-C., MORTIER L., DAESELEIRE E., FODEY T., ELLIOTT C., DELAHAUT P.
Screening for the coccidiostats halofuginone and nicarbazin in egg and chicken muscle : development of an ELISA.
Food Additives and Contaminants, February 2005, 22(2), 128-134.

MORTIER L., HUET A.-C., CHARLIER C., DAESELEIRE E., DELAHAUT P., VAN PETEGHEM C.
Incidence of residues of nine anticoccidials in eggs.
Food Additives and Contaminants, 2005, 22(11), 1120-1125.

MORTIER L., HUET A.-C., DAESELEIRE E., HUYGHEBAERT G., FODEY T., ELLIOTT C., DELAHAUT P., VAN PETEGHEM C.
Deposition and depletion of five anticoccidials in eggs.
J. Agric. Food Chem., 2005, 53, 7142-7149.

THEVIS M., THOMAS A., DELAHAUT P., BOSSELOIR A., SCHÄNZER W.
Qualitative determination of synthetic analogues of insulin in human plasma by immunoaffinity purification and liquid chromatography - tandem mass spectrometry for doping control purposes.
Anal. Chem., 2005, 77, 3579-3585.

COOPER J., DELAHAUT P., FODEY T.L., ELLIOTT C.T.
Development of a rapid screening test for veterinary sedatives and the beta-blocker carazolol in porcine kidney by ELISA.
Analyst, 2004, 129, 169-174.

DELAHAUT P., BRASSEUR P.-Y., DUBOIS M.
Multiresidue method for the detection of tranquillisers, xylazine, and a ß-blocker in animal production by liquid chromatography-tandem mass spectrometry.
Journal of Chromatography A, 2004, 1054, 373-378.

DUBOIS M., PIERRET G., DELAHAUT P.
Efficient and sensitive detection of residues of nine coccidiostats in egg and muscle by liquid chromatography-electrospray tandem mass spectrometry.
Journal of Chromatography B, 2004, 813, 181-189.

MORTIER L., DAESELEIRE E., HUET A.-C., DELAHAUT P., VAN PETEGHEM C.
Determination of halofuginone in eggs by liquid chromatography/tandem mass spectrometry after cleanup with immunoaffinity chromatography.
Rapid Commun. Mass Spectrom., 2004, 18, 1817-1820.

BEN REJEB S., ABBOTT M. DAVIES D., QUERRY J., CLEROUX C., STRENG C., DELAHAUT P., YEUNG J.M.
Immunochemical-based method for detection of hazelnut proteins in processed foods.    
Journal of AOAC International, 2003, 86, 557-563.

DELAHAUT P., LEVAUX C., ELOY P., DUBOIS M.
Validation of a method for detecting and quantifying tranquillisers and a beta-blocker in pig tissues by liquid chromatography-tandem mass spectrometry.
Analytica Chimica Acta, 2003, 483, 335-340.

FODEY T.L., CONNOLLY L., CROOKS S.R.H., DELAHAUT P., ELLIOTT C.T.
Production and characterisation of polyclonal antibodies to a range of nitroimidazoles.
Analytica Chimica Acta, 2003, 483, 193-200.

MORTIER L., DAESELEIRE E., DELAHAUT P.
Simultaneous detection of five coccidiostats in eggs by liquid chromatography-tandem mass spectrometry.
Analytica Chimica Acta, 2003, 483, 27-37.

DELAHAUT P., LEVAUX C., FLUCHARD D., PIERRE J., DUBOIS M.
Détection par dosage immunologique et spectrométrie de masse couplée à la chromatographie liquide des principaux tranquillisants utilisés chez le porc.
Nouvelles méthodes analytiques pour la détection de substances à activité hormonale et de tranquillisants en production animale.
Ministère des Classes moyennes et de l'Agriculture, Administration Recherche et Développement DG6, Service Recherche subventionnée, 2002, pages 114-139.

DESROCHES M.C., MATHURIN J.C., RICHARD Y., DELAHAUT P., DE CEAURRIZ J.
Urinary 19-norandrosterone purification by immunoaffinity chromatography : application to gas chromatography/combustion/isotope ratio mass spectrometry analysis.
Rapid Commun. Mass Spectrom., 2002, 16, 370-374.

ECTORS F.J., DELFOSSE H., DE WEDER L., ZWAENEPOEL P., DELAHAUT P.
Embryo transfer as a method for eliminating pathogenic agents in a rabbit colony.
Vlaams Diergeneeskundig Tijdschrift, 2002, 71, 334-339.

GUILLAUME G., LEPAGE S., DUYCKAERTS A., MATTON A.-M., DEGAND G., FRERE J.-M., GRANIER B., SCIPPO M.-L., MAGHUIN-ROGISTER G., DUBOIS M., PIERRET G., DELAHAUT P.
Mise au point de tests rapides (récepteur essais) permettant la détection des principaux antibiotiques dans la viande et leur confirmation par analyse physico-chimique (LC-MS/MS).
Ministère des Classes moyennes et de l'Agriculture, Administration Recherche et Développement DG6, Service Recherche subventionnée, 2002, pages 1-42.

POELMANS S., DE WASCH K., DE BRABANDER H.F., VAN DE WIELE M., COURTHEYN D., VAN GINKEL L.A., STERK S.S., DELAHAUT P., DUBOIS M., SCHILT R., NIELEN M., VERCAMMEN J., IMPENS S., STEPHANY R., HAMOIR T., POTTIE G., VAN POUCKE C., VAN PETEGHEM C.
Analytical possibilities for the detection of stanozolol and its metabolites.
Analytica Chimica Acta, 2002, 473, 39-47.

BOBIN S., POPOT M.A., BONNAIRE Y., PIRENS G., CLOSSET J., DELAHAUT P., TABET J.C.
High performance liquid chromatography / Ion trap mass spectrometry for the determination of Insulin like growth factor-I in horse plasma.
Chromatographia, 2001, 54, 737-741.

DUBOIS M., FLUCHARD D., SIOR E., DELAHAUT P.
Identification and quantification of five macrolide antibiotics in several tissues, eggs, and milk by liquid chromatography-electrospray tandem mass spectrometry.
Journal of Chromatography B, 2001, 753, 189-202.

POPOT M.A., BOBIN S., BONNAIRE Y., DELAHAUT P., CLOSSET J.
IGF-I plasma concentrations in non treated horses and horses administered with methionyl equine somatotropin.
Research in Veterinary Science, 2001, 71, 167-173.

FLUCHARD D., KIEBOOMS S., DUBOIS M., DELAHAUT P.
Determination of a method for detecting and quantifying azaperone, azaperol and carazolol in pig tissues by liquid chromatography-tandem mass spectrometry.
Journal of Chromatography, 2000, 744, 139-147.

BiomedHelp project

Title: Assistance in the development of diagnostics and quality control for the biomedical sector

Project developer:        CER Group – Health Department

Acronym:                         BiomedHelp

Contract no. :                  1318120

From:                               01/01/2014

To:                                    31/12/2015

Logos :

dgo6                 logo SPF logo CERTECH 


  ImmuneHealth-logo  

 

Financed by: regional level:
Wallonia Region – Directorate General Operational of Economy, Employment and Research (DGO6)


Brief outline of the project:

On 1 January 2014, we launched our new technological guide “BiomedHelp”, which is intended to support enterprises operating in the field of health and, more specifically, those involved with biotechnologies and the pharmaceutics sector.

The guide has four main objectives:

  1. to support manufacturers and researchers in their research related to the development of in vitro diagnostics;
  2. to offer quality reagents and/or tools to enhance and increase diagnostics tests in the healthcare chain;
  3. to help determine new biomarkers by guaranteeing access to the necessary biological human samples;
  4. to develop analytical tools for the quality control of drugs.


Partners:

projet BiomedHelp

Role of the CER:

  • Customised service for the production and characterisation of antibodies
  • Purification of antibodies in line with needs of the customer
  • Analyses of drug samples or matrices containing the drug
  • Identification of constraints and problems encountered and solutions to remedy them  
  • Sharing our experience in biochemistry and biology
  • Monitoring of scientific literature and documentation

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 For more information, please contact us:

CER Group – Health Department

Anne-Catherine HUET
+32 (0) 84 31 00 90
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Dr Ph. DELAHAUT
+32 (0) 84 31 00 90
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CONffIDENCE project

Title: Contaminants in food and feed: Inexpensive detection for control of exposure

Acronym: CONffIDENCE

Agreement no.: 21132


Logo:

    logo projet conffidence    Nanolyse Seventh Framework Programme      logo CEE   

 

Financed by: European Community– Seventh Framework Programme

Brief project summary:
     
One of the key concerns of European governments and consumers is the presence of chemical contaminants in foodstuffs.
Consequently, the regulatory bodies and the food products industries have devoted considerable budgets to regulating and controlling the safety of human and animal foods. This regulation often requires expensive methods that can only detect a single chemical substance. That is why it is urgent to replace the present methods by validated screening tools that are simple, inexpensive, rapid and which are capable of detecting in parallel as many chemical contaminants as possible.
The CONffIDENCE project was set up in order to provide long-term solutions for investigating a wide variety of chemical contaminants. Those include pesticides, persistent organic pollutants, veterinary drugs such as antibiotics, heavy metals, toxins originating in plants and crustaceans and mycotoxins. Tests will be developed and validated for products such as food for fish, cereal products and vegetables.
A harmonious equilibrium between the new technologies will be sought. These will range from dipstick tests that can be used like a pregnancy test and from inexpensive to high performance laboratory methods. After optimisation, these simplified methods will be used in order to perform international food investigations which will contribute to assessing the exposure of consumers to chemical contaminants. In addition, the tests will be used to study the transfer of harmful contaminants from feed to eggs and meat.

Partners:

Partenaires CONffIDENCE

Role of the CER:

The CER Group is involved in five working groups:

  • WP2a: Assistant to the manager for this working group on coccidiostats. Production of polyclonal antibodies, evaluation of immunochemical reagents by ELISA, production of processed eggs and support with the validation of screening methods and by LCMS.
  • WP2b: Antibiotics. Production of polyclonal antibodies, assay of immunochemical reagents by ELISA, produced from urine and processed pork muscle. In addition, support for the validation of screening methods and by LCMS.
  • WP4a: Alkaloids. Production of polyclonal antibodies, evaluation of immunochemical reagents by ELISA.
  • WP4b: Marine biotoxins. Production of polyclonal antibodies, evaluation of immunochemical reagents by ELISA.
  • WP4c: Assistant to the manager for the working group on mycotoxins. Production of polyclonal antibodies, evaluation of immunochemical reagents by ELISA.

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For more information, visit the official CONffIDENCE site: http://www.conffidence.eu or contact us:

CER Group - Health Department

Anne-Catherine HUET
+32 (0)84 31 00 90
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Philippe DELAHAUT
+32 (0)84 31 00 90
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Poultry-Check project (2000-01-02 -> 2003-07-31)

Title: Multi-residue screening for coccidiostatic compounds used in poultry production

Acronym : Poultry-Check (EU-FP6-FOOD)

Finance : EU (Contract no.: QLK1-CT-1999-00313)

Partners :

Participant No.1 Department of Biotechnology, University of Turku (co-ordinator, associated with ITD)
Person responsible Prof. Timo Lövgren
Mailing address BioCity 6A, FIN-20520 Turku, Finland

 

Participant N.2 Inno Trac Diagnostrics Oy, Finland (Associated contractor to TU)
Person responsible Harri Takalo
Mailing adress Kalevantie 25, 20520 Turku, Finland

 

Participant No.3 Queen's University of Belfast, Department of Veterinary Sciences (Contractor, associated  with Health Department, RIKILT and SLV)
Person responsible Chris Elliott
Mailing address Department of Veterinary Sciences, Stoney Road, Belfast BT4 3SD, UK

 

Participant No.4 CER Groupe, Health Department (Associated contractor to QUB)
Person responsible Philippe Delahaut
Mailing address CER Groupe, Health Department, rue du Carmel 1, 6900 Marloie, Belgium

 

Participant No.5 State Institute for Quality control of Agricultural products (Associated contractor to QUB)
Person responsible Hans van Rhijn
Mailing address RIKILT-DLO, P.O. Box 230, Bornsesteeg 45, 6700 AE, Wageningen, The Netherlands

 

Participant No.6 The National Food Administration (Associated contractor to QUB)
Person responsible Gunnel Alfredsson
Mailing address Livsmedelsverket/Chemistry Division 1, PO Box 622, SE 75126, Uppsala, Sweden

 

Work Package 1 : Antibody development

Summary of the completed project (project output) :

The role of the CER consisted essentially of producing the different antibodies needed for the development of immunoassays.
EU legislation demands that residues of xenobiotics used in poultry production to prevent and treat disease are monitored. However there is strong evidence that some coccidiostat residues (suspect carcinogens) are present in meat and the consumer is not being given adequate protection. The project objective is to develop a simple, user friendly and automated immunoassay system for broad spectrum screening of the important coccidiostat residues. A novel time-resolved fluorescence based detection system employing dry chemistry and intrinsically fluorescent labels, already successful in hyman diagnostic applications, will be utilised. Confirmatory assays will also be developed for these residues. National Reference Labs (end-users) will be trained through Technology Transfer events to use the methods, thus improving harmonisation of testing. The 2 SME partners can commercialise high quality diagnostic products as a result of the project.

Logo:

logo projet Poultry-check

BioCop project (2005-01-01 --> 2010-09-30)

Title : New Technologies to Screen Multiple Chemical Contaminants in Foods

Acronym : BioCop

Finance: EU-FP6-FOOD

Partenaires : 33
1.     Queen’s University Belfast (UK)  
2.     RIVM (EU-CRL on Residues) (NL)
3.     Biacore AB (S)
4.     University of Santiago de Compostela (E)
5.     University of Zurich (CH)
6.     Xenosense Ltd. (UK)
7.     RIKILT Institute of Food Safety (NL)
8.     National Veterinary School (F)
9.     CER Groupe - Département Santé (B)
10.    University of Turku (FIN)
11.    Institute of Chemical Technology (CZ)
12.    Joint Research Centre, Institute for Reference Materials and Measurements (B)
13.    University of Utrecht (NL)
14.    Nestec Ltd. (Nestlé Research Centre) (CH)
15.    Central Science Laboratory (UK)
16.    Università di Roma Tor Vergata (I)
17.    University of Natural Resources and Applied Life Sciences (A)
18.    Fusion Antibodies Ltd. (UK)
19.    Institute of Biochemistry (LT)
20.    The Food Consultancy (dmc) Ltd. (UK)
21.    Eurofins/Wiertz-Eggert-Jörissen GmbH (D)
22.    Université de Liège, Centre d’Analyse des Résidus en Traces (B)
23.    Clondiag GmbH (D)
24.    Genedata AG (CH)
25.    ANFACO (E)
26.    Community Reference Laboratory for Marine Biotoxins, Vigo (E)
27.    Palm Instruments BV (NL)
28.    Biopure (A)
29.    AFSSA (F)
30.    Swedish University of Agricultural Sciences (S)
31.    National Food Centre (IRL)
32.    Chalex Research Ltd. (UK)
33.    Health Canada (CA)
 

Work Packages :

  • WP 2 : Proteomics (P’OMICS)
  • WP 4 : Binding molecules (BINDERS)
  • WP 6 : Pesticides (P’CIDES)
  • WP 8 : Therapeutics (T’PEUTICS)

Summary of the completed project (project output):

The BioCop project is a European project based on new technologies to detect the presence of multiple chemical contaminants in food. The CER Group was involved in three working groups, WP2 (proteomics), WP4 (binding molecules) and WP8 (therapeutics). Our objective within WP2 was to help with the identification of biomarkers following the abusive use of anabolic agents in animal husbandry. Our objective within WP4 consisted of producing antibodies (polyclonal and/or monoclonal) to identify chemical contaminants predefined by the other partners. We succeeded in developing a screening method that enabled 11 fluoroquinolones (antibiotics) to be detected simultaneously in fish, poultry meat and eggs. This method, based on biosensors, exploits the principle of Surface Plasmon Resonance (SPR) and has been fully validated according to European criteria.



Logo:
logo projet BioCop
http://www.biocop.org

 

Completed regional project: Walreach

Assistance to Walloon enterprises as part of the REACH directive
SPW, Operational Central Office for Economy, Employment and Research
Technical support
CER: Sponsor

Agreement no. 917026

2010–2011

Completed regional project: BioPhar

Advanced biological treatment for waste water contaminated with Pharmaceutical Products (PPs)
SPW, Central Office of Technologies, Research and Energy (DGTRE)
"Collective Research" programme
CER: Partner

Agreement no. 816949

2009–2011

Completed regional project: Allergen

Follow-up of contamination with food allergens for the Wallonia agro-food industry
SPW, Operational Central Office for Economy, Employment and Research
Technical support
CER: Sponsor

Agreement no. 516274-0301014

2006 - 2009

Completed regional project: Mycotox

Follow-up of mycotoxin contamination in the brewing industry and food manufacture in order to improve manufacturing processes
SPW, Operational Central Office for Economy, Employment and Research
Technical support
CER: Sponsor

Agreement no. 516273 - 001014

2006–2009

Completed national project: Respoul

Development of a model for the determination of transfer factors of residues in poultry.
SPF Public Health, Safety of the food chain and environment
Contractual research
CER: Sponsor

 

Project RT07/1

2008 - 2011

Completed national project: Detall

Standardisation and validation of different methods of analysing allergens available in the form of commercial tests in prepackaged food.  Support for the development of a management strategy.
SPF Public Health, Safety of the food chain and environment
Contractual research
CER: Sponsor

Project RT06/10

2007 - 2009

Completed national project: Alteval1

Validation of ELISA and spectrometric techniques for the detection of phycotoxins to replace biological tests on mice.
SPF Public Health, Safety of the food chain and environment
Contractual research
CER: Sponsor

Project RT06/5

2007 - 2008

VEGEPROT project

Title: Development of the formulation of plant proteins in nutrition

Acronym:  VEGEPROT

Agreement no.: CWAlity – no. 111 74 38

Logo:

dgo6logo SPF


Financed by: Regional level: Wallonia Region – DGO6 - CWAlity (no. 111 74 38)


Brief project summary:

As part of research into new sources of proteins for human foodstuffs, Biohainaut is suggesting hydrolysing some plants in order to improve their digestibility or to reduce their allergenicity. The role of the CER will be to provide suitable models with which to evaluate the health benefits of hydrolysing these proteins.

Partners:

partenaires Vegeprot


Role of the CER:

The CER is involved in 6 work packages of the project:

  • WP6: creation of a model to evaluate the efficacy of a protein incorporated widely in food
  • WP7: determination of the digestibility of the proteins
  • WP8: determination of the digestibility of the hydrolysates
  • WP9: determination of the acute and chronic toxicity of the lytic enzymes together with the hydrolysates
  • WP10: evaluation of the reduction of the allergenic potential
  • WP11: Report and conclusion  

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Contact us for more information:

CER Group - Health Department

Eric FICHANT
+32 (0)84 31 00 90
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Philippe DELAHAUT
+32 (0)84 31 00 90
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CYTOFOOD project

Title: Rapid detection, by flow cytometry, of allergens and chemical contaminants in foodstuffs

Acronym:  CYTOFOOD

Agreement no.: 1217554

Logo:

dgo6logo SPF


Financed by: Regional level: Wallonia Region – DGO6 - technical support agreement

Brief project summary:

The principal objective of the CYTOFOOD project is to make basic and rapid multi-detection tools available to the agrofood sector to check for contaminants. To do this, the project uses an immunological technique of multi-compound detections, based on the use of microspheres and their characterisation by flow cytometry. Using this technique, a large number of substances can be detected from a low sample volume.

Current developments are enabling us to envisage the possibility of the company being able to perform analyses itself using a portable device that represents a reasonable investment.

The outcome of the project will be that the businesses being targeted in the agrofood sector will have at their disposal, via the centres involved in CYTOFOOD, the necessary expertise to adapt specific tests for each sphere of activity. All the reagents the companies need to perform their analyses will be accessible via the two partner research centres.

If the company does not want to invest in a cytometer, it will be always be possible to perform the analyses via a laboratory that does have the technical equipment.

Partners:

Projet CYTOFOOD tableau3

Role of the CER: The role of the CER is to

  • Develop antibodies to the compounds of interest (allergens and chemical substances)
  • Characterise these antibodies
  • Develop multi-compound detection tests by flow cytometry
  • Transfer the detection kits on site

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Contact us for more information:

CER Groupe - Département Santé

Valéry DUMONT
+32 (0)84 31 00 90
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Philippe DELAHAUT
+32 (0)84 31 00 90
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